Dr. Jianjun Li

Human Health Therapeutics, National Research Council Canada, Ottawa, Canada

Mass spectrometry techniques for vaccine development – analysis of bacterial antigens and cancer neoantigens

Abstract

In this presentation, I will first discuss a CE-MS technique developed for the analysis of lipooligosaccharide (LOS) from Gram-negative bacteria. We were able to obtain the structural information of bacterial surface carbohydrates using material isolated from as little as one colony. I will also present method development for structural characterization and quantification of endotoxin (lipid A). In the last part of my presentation, I will discuss the development and application of a novel chemical derivatization strategy to expand the coverage of the MHC peptidome.

Biosketch

Dr. Jianjun Li is a Senior Research Officer and Team Lead of Bioanalysis at Human Health Therapeutics of National Research Council (NRC-HHT). He received his B. Sc. in Chemistry and Ph. D. in Analytical Chemistry from Wuhan University, China. Dr. Li’s research has involved the development of high-resolution separation techniques coupled with mass spectrometry and their applications to the structural characterization of various biological molecules. From 1997 to 2001, he focused on the development of chip-based capillary electrophoresis-mass spectrometry (Chip-CE/MS) systems. His current research interest is method development and application for bacterial vaccine and cancer vaccine, including the structure and biosynthesis of bacterial surface carbohydrates and cancer neoantigen presented by major histocompatibility complex (MHC). He is author or co-author of over 200 original peer-reviewed scientific papers, 8 book chapters and edits the book entitled Methods in Functional Glycomics.

Date
Date(s) - September 26, 2017
6:00 pm - 9:00 pm

Emplacement / Location
Morris and Rosalind Goodman Agora