Research Scientist Waters Corporation
Development and application of a cyclic ion mobility mass spectrometer
Improvements in the performance and availability of commercial instrumentation have made ion mobility – mass spectrometry (IM-MS) an increasingly popular approach for the structural analysis of ionic species as well as for separation of complex mixtures. Here, a new research instrument is presented which enables complex experiments, extending the current scope of IM technology. The instrument is based on a Waters SYNAPT G2-Si IM-MS platform, with the IM separation region modified to accept a cyclic ion mobility (cIM) device. In addition to single and multi-pass separations around the cIM, providing selectable mobility resolution, the instrument design and control software enable a range of ‘multi-function’ experiments such as: mobility selection, activation, storage, IMSn and importantly, custom combinations of these functions. In this presentation, instrument performance, functionality and flexibility will be showcased in a study of isomeric pentasaccharides. Using high cIM resolution we demonstrate the separation of three isomeric pentasaccharides and, moreover, that three components are present for each compound. Using IMSn we show that structural differences between product ions reflect the precursor differences in some cases but not others. These findings are corroborated by a heavy oxygen labelling approach. Using this methodology, the identity of fragment ions may be assigned. This enables us to postulate that the two main components observed for each pentasaccharide are anomeric forms. The remaining low abundance component is assigned as an open-chain form.
Jakub Ujma graduated from Edinburgh University with a degree in Chemistry with Industrial Experience (2012), and from Manchester University with a PhD in Chemistry (2016) under the supervision of Professor Perdita Barran. During his PhD research he built a variable temperature (150-500K) ion mobility drift tube, coupled to a commercial Q-ToF mass spectrometer. Jakub joined Waters MS research team in 2016 where he works on the development of cyclic ion mobility technology.
Date(s) - December 10, 2019
6:00 pm - 9:00 pm
Emplacement / Location
Université de Montréal - Pavillon Jean Coutu (S1-151)