Dr. Jianjun Li
Human Health Therapeutics, National Research Council Canada, Ottawa, Canada
Mass spectrometry techniques for vaccine development – analysis of bacterial antigens and cancer neoantigens
In this presentation, I will first discuss a CE-MS technique developed for the analysis of lipooligosaccharide (LOS) from Gram-negative bacteria. We were able to obtain the structural information of bacterial surface carbohydrates using material isolated from as little as one colony. I will also present method development for structural characterization and quantification of endotoxin (lipid A). In the last part of my presentation, I will discuss the development and application of a novel chemical derivatization strategy to expand the coverage of the MHC peptidome.
Dr. Jianjun Li is a Senior Research Officer and Team Lead of Bioanalysis at Human Health Therapeutics of National Research Council (NRC-HHT). He received his B. Sc. in Chemistry and Ph. D. in Analytical Chemistry from Wuhan University, China. Dr. Li’s research has involved the development of high-resolution separation techniques coupled with mass spectrometry and their applications to the structural characterization of various biological molecules. From 1997 to 2001, he focused on the development of chip-based capillary electrophoresis-mass spectrometry (Chip-CE/MS) systems. His current research interest is method development and application for bacterial vaccine and cancer vaccine, including the structure and biosynthesis of bacterial surface carbohydrates and cancer neoantigen presented by major histocompatibility complex (MHC). He is author or co-author of over 200 original peer-reviewed scientific papers, 8 book chapters and edits the book entitled Methods in Functional Glycomics.
Date(s) - 26/09/2017
6:00 pm - 9:00 pm
Emplacement / Location
Morris and Rosalind Goodman Agora
Groupe de discussion en spectrométrie de masse de Montréal (GDSMM)
Le groupe de discussion sur la spectrométrie de masse de Montréal (GDSMM) est un organisme à but non lucratif qui offre un forum favorisant les interactions locales, nationales et internationales entre les scientifiques oeuvrant en spectrométrie de masse. Le GDSMM organise des séminaires scientifiques et des forums de discussion présentant les derniers développements en recherche fondamentale et appliquée en spectrométrie de masse. Une réunion annuelle du GDSMM qui regroupe les étudiants et les post-doctorants a lieu à Montréal chaque printemps et offre une opportunité unique de réseautage en plus d’accroitre la visibilité et la sensibilisation aux contributions locales en spectrométrie de masse.
Montreal mass spectrometry discussion group (MMSDG)
The Montreal mass spectrometry discussion group (MMSDG) is a not-for-profit organization that provides a forum to promote local, national and international interactions among scientists involved in mass spectrometry. MMSDG sponsors scientific seminars and forums to present the latest developments in basic and applied research in mass spectrometry. An annual MMSDG meeting that regroups students and postdoc is held every spring in Montreal, and provide a unique networking opportunity to participants and enhance the visibility and awareness of local contributions in mass spectrometry.